Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Visibility: Public
Publication: Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary Link
Journal: BioTechniques
Source: NA
Sample Number: 562

Samples in this project
  

SampleID Type Habitat Year Sequence type MNS1 MAS2
S_11176.1x.2.BLANK.H12 Freshwater NA 2017 16S V4 - -
S_11176.1x.2.Dust.A12 Building NA 2017 16S V4 - -
S_11176.1x.2.Dust.B12 Building NA 2017 16S V4 - -
S_11176.1x.2.Dust.C12 Building NA 2017 16S V4 - -
S_11176.1x.2.Dust.D12 Building NA 2017 16S V4 - -
S_11176.1x.2.Dust.E12 Building NA 2017 16S V4 - -
S_11176.1x.2.Dust.F12 Building NA 2017 16S V4 - -
S_11176.1x.2.MBQC.A1 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.A2 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.A3 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.A4 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.A5 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.A6 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.B1 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.B3 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.B4 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.B5 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.B6 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.C1 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.C3 Gut Gut 2017 16S V4 - -

1MNS (Microbiome Novelty Score): < 0.12: Non-novel; 0.12-0.2: Novel; > 0.2: Very novel; -: NA for samples before 2010

2MAS (Microbiome Attention Score): < 10: Low-attention; ≥ 10: High-attention; -: NA for samples before 2010