Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Visibility: Public
Publication: Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary Link
Journal: BioTechniques
Source: NA
Sample Number: 562

Samples in this project
  

SampleID Type Habitat Year Sequence type MNS1 MAS2
S_11176.1x.2.MBQC.F6 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.G1 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.G2 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.G4 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.G5 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.G6 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.H1 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.H3 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.H4 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.H5 Gut Gut 2017 16S V4 - -
S_11176.1x.2.MBQC.H6 Gut Gut 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.A9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.B9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.C9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.D9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.E8 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.E9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.F8 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.F9 Marine NA 2017 16S V4 - -
S_11176.1x.2.Marine.Sediment.G8 Marine NA 2017 16S V4 - -

1MNS (Microbiome Novelty Score): < 0.12: Non-novel; 0.12-0.2: Novel; > 0.2: Very novel; -: NA for samples before 2010

2MAS (Microbiome Attention Score): < 10: Low-attention; ≥ 10: High-attention; -: NA for samples before 2010