Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

Visibility: Public
Publication: Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary Link
Journal: BioTechniques
Source: NA
Sample Number: 562

Samples in this project
  

SampleID Type Habitat Year Sequence type MNS1 MAS2
S_11176.1x.2.Metcalf.Soil.H7 Soil NA 2017 16S V4 - -
S_11176.1x.2.Oral1.D11 Oral Oral 2017 16S V4 - -
S_11176.1x.2.Oral1.E11 Oral Oral 2017 16S V4 - -
S_11176.1x.2.Oral1.F11 Oral Oral 2017 16S V4 - -
S_11176.1x.2.Oral2.G11 Oral Oral 2017 16S V4 - -
S_11176.1x.2.Oral3.H11 Oral Oral 2017 16S V4 - -
S_11176.1x.2.Skin1.A11 Skin Skin 2017 16S V4 - -
S_11176.1x.2.Skin1.G10 Skin Skin 2017 16S V4 - -
S_11176.1x.2.Skin1.H10 Skin Skin 2017 16S V4 - -
S_11176.1x.2.Skin2.B11 Skin Skin 2017 16S V4 - -
S_11176.1x.2.Skin3.C11 Skin Skin 2017 16S V4 - -
S_11176.1x.2.Vibrio.G12 Other_environment NA 2017 16S V4 - -
S_11176.1x.4.BLANK.H12 Freshwater NA 2017 16S V4 - -
S_11176.1x.4.Dust.A12 Building NA 2017 16S V4 - -
S_11176.1x.4.Dust.B12 Building NA 2017 16S V4 - -
S_11176.1x.4.Dust.C12 Building NA 2017 16S V4 - -
S_11176.1x.4.Dust.D12 Building NA 2017 16S V4 - -
S_11176.1x.4.Dust.E12 Building NA 2017 16S V4 - -
S_11176.1x.4.Dust.F12 Building NA 2017 16S V4 - -
S_11176.1x.4.MBQC.A1 Gut Gut 2017 16S V4 - -

1MNS (Microbiome Novelty Score): < 0.12: Non-novel; 0.12-0.2: Novel; > 0.2: Very novel; -: NA for samples before 2010

2MAS (Microbiome Attention Score): < 10: Low-attention; ≥ 10: High-attention; -: NA for samples before 2010